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Nature Ecology & Evolution

Stephanie Pierce, Katrina Jones, Sarah Gonzalez (Harvard University), Kenneth Angielczyk (Field Museum of Natural History)

Harvard University

Posted by Anna Ivanova
Anna covers archaeology, paleontology, and anthropology.
Contact: archaeology@sciglow.com

How and when spines changed in mammalian evolution

Harvard-led study shows what drives functional diversity in mammalian spines.

3 weeks ago by Harvard University

From the long necks of giraffes that help them reach treetops to the flexibility of a cheetah’s spine that help it go from zero to sixty in three seconds, mammals not only have the figurative backbone to do amazing things but, more importantly, they have the literal one. But they weren’t always that way.

A new study published in Nature Ecology and Evolution by Harvard researchers and collaborators at the Field Museum of Natural History sheds light on how and when changes in the spine happened in mammal evolution. The research shows how a combination of developmental changes and adaptive pressures in the spines of synapsids, the extinct forerunners of mammals, laid the groundwork for the diversity of backbones seen in mammals today.

Illustration of Dimetrodon, pelycosaur synapsid, showing the elaborate backbone sail. This study shows that despite their bizarre sails, it is likely that their vertebral movements were relatively uniform along their back, more similar to living lizards or salamanders than to mammals. Credit: 2019 Mark Witton

Using a novel combination of techniques, including biomechanical experimentation on the cadavers of two modern animals, cat and lizard, and CT scans of synapsid fossils, the researchers overturned the traditional notion that the gradual accumulation of different regions (or independent sections) of the spine alone account for its evolving complexity. New evidence suggests that regions (like the thorax and lower back) evolved long before new spinal functions, such as bending and twisting. The study points to the idea that the right selective pressures or animal behaviors combined with these already existing regions played a significant role in the evolution of their unique functions.

The study, led by Stephanie Pierce, Associate Professor of Organismic and Evolutionary Biology and Curator of Vertebrate Paleontology and Katrina Jones, postdoctoral researcher, taps into the larger question of how mammals, including humans, evolved over millions of years. Mammals today, for instance, have developed compartmentalized spinal regions that take on a number of diverse shapes and functions without affecting other spinal regions. This has allowed the animals to adapt to different ways of life, explained Jones.

Flesh reconstruction of Thrinaxodon, a cynodont synapsid, showing the different regions of the backbone evolving. This study shows that regions appear in the ancestors of mammals with little change in function, implying an adaptive trigger was necessary to exploit the regions for different functions. Credit: 2018 April Isch Neander.

In a previous study the authors showed that extinct pre-mammalian land animals developed these small but distinct regions during evolution.

“What we were able to show in 2018, was that even though all the vertebrae looked very similar in early mammal ancestors they had subtle differences and those subtle differences created distinct developmental regions,” Pierce said. “What we’re showing with this new study is that those distinct regions were really important as they provided the raw material that facilitated functional differentiation to happen. Basically, if you don’t have these distinct developmental regions in place and you have a selective pressure all the vertebrae are going to adapt in the same way.”

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It’s long been thought that developing different spinal regions is one important step in evolving backbones with many functions, but Pierce and Jones show that this isn’t enough. An evolutionary trigger was also required, in this case the evolution of a highly active lifestyle that put new demands on the backbone.

Jones said, “We’re trying to get at something that’s quite a fundamental evolutionary question which is: How does a relatively simple structure evolve into a complex one that can do lots of different things? Is that determined by the limitations of development or natural selection related to the behavior of the animal?”

The researchers compared the spine of two animals essentially on opposite ends of the evolutionary and anatomical spectrum: a cat, which has highly developed spinal regions, and a lizard, which has a pretty uniform backbone. They looked at how each animal’s spinal joints bent in different directions to measure how the form of the vertebrae reflects their function.

Exhibit specimen of Edaphosaurus, a pelycosaur synapsid, from the collections at the Museum of Comparative Zoology. Pelycosaurs are the most ancient forerunners of mammals. This study shows that despite their bizarre sails, it is likely that their vertebral movements were relatively uniform along their back, more similar to living lizards or salamanders than to mammals. Credit: Harvard University Museum of Comparative Zoology

Determining that while some spinal regions can function differently from one to the other, others don’t. For example, the lizard’s backbone comprised several distinct regions but they all acted in the same way.

The researchers, including Kenneth Angielczyk of the Field Museum of Natural History, then turned their focus to finding out when different regions started taking on different functions in the evolution of mammals. Using cat and lizard data showing that if two joints in the spine looked different they tended to have different functions, they mapped out how spinal function in those fossils changed through time.

“The earliest ancestors of mammals have a remarkably good fossil record considering that those animals lived between about 320 and 250 million years ago,” Angielczyk said.

Researchers found, despite having the developmental regions capable of performing different functions, the level of functional variation seen in mammals today didn’t start to take hold until late in synapsid evolution.

“We then hypothesized that maybe it was the evolution of some new mammalian behaviors that helped trigger this [in these late synapsids] and provided the natural selection that could exploit the regions that were already there,” Jones said.

Their findings fit with the fact that the group in which this functional diversity occurs — the cynodonts, which directly preceded mammals — have a number of mammalian features, including evidence they could breathe like a mammal. The researchers believe that these mammal-like features shifted the job of breathing away from the backbone and ribs to the newly-evolved diaphragm muscle, releasing the spine from an ancient biomechanical constraint. This enabled the backbone to adapt to interesting new behaviors, such as grooming fur, and take on new functions.

Pierce and Jones do not yet know what those functions looked like in these extinct animals, which is what they plan to focus on in future research.

“It’s definitely not the end of the story,” Jones said.